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Vimentin Antibody (S82)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - Vimentin Antibody (S82) AP2739a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling Vimentin with AP2739a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplaasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 1 - Vimentin Antibody (S82) AP2739a
    All lanes : Anti-Vimentin Antibody (S82) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Vimentin Antibody (S82) AP2739a
    Western blot analysis of Vimentin-S82 (Cat. #AP2739a) in Hela cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.
  • 1 - Vimentin Antibody (S82) AP2739a
    Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) (Cat.#AP2739a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
  • 14 - Vimentin Antibody (S82) AP2739a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) (Cat.#AP2739a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, IHC-P, E
Primary Accession P08670
Other Accession P31000, P20152, Q4R4X4, P48670, P48616
Reactivity Human, Mouse
Predicted Rat, Bovine, Hamster, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVimentin Antibody (S82) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas

BACKGROUND

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

REFERENCES

References for protein:
1.Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
2. Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
3. Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]

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