- 文献引用 : 4
|Application ||WB, E|
|Calculated MW||54671 Da|
|Other Names||Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF|
|Target/Specificity||This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-53 amino acids from the N-terminal region of human KLF4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KLF4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.|
Author : Xu L1,Sun H2,Zhang M2,Jiang Y2,Zhang C2,Zhou J2,Ding L2,Hu Y3,Yan G4.
Mol Cell Endocrinol. 2017 May 28. pii: S0303-7207(17)30299-X. doi: 10.1016/j.mce.2017.05.030. [Epub ahead of print]
Author : Yan C1, Yu J2, Liu Y3, Kang W1, Ma Z1, Zhou L1.
Biochem Biophys Res Commun. 2015 Oct 12. pii: S0006-291X(15)30744-0. doi: 10.1016/j.bbrc.2015.10.044. [Epub ahead of print]
Author : Jumabay M1, Abdmaulen R, Ly A, Cubberly MR, Shahmirian LJ, Heydarkhan-Hagvall S, Dumesic DA, Yao Y, Boström KI.
Stem Cells Transl Med. 2014 Feb;3(2):161-71. doi: 10.5966/sctm.2013-0107. Epub 2014 Jan 6.
Author : Marini MG, Porcu L, Asunis I, Loi MG, Ristaldi MS, Porcu S, Ikuta T, Cao A, Moi P.
Br J Haematol. 2010 Jun;149(5):748-58. doi: 10.1111/j.1365-2141.2010.08130.x. Epub 2010 Mar 15.
Provided below are standard protocols that you may find useful for product applications.
Kruppel-like factor 4 (KLF4) is a transcription factor involved in both proliferation and differentiation in the colon. It is down-regulated in both mouse and human colonic adenomas and has been implicated as a tumor suppressor in the gut, whereas in breast cancer, KLF4 is an oncogene. KLF4 is also involved in reprogramming differentiated cells into pluripotent stem cells. KLF4 can act as a transcriptional activator or repressor, but the underlying mechanisms are poorly understood.
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