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WIF1 Antibody(Human C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - WIF1 Antibody(Human C-term) AP2723b
    Immunohistochemical analysis of paraffin-embedded human colon tissue using AP2723b performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - WIF1 Antibody(Human C-term) AP2723b
    Anti-WIF1 Antibody(Human C-term) at 1:2000 dilution + Human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - WIF1 Antibody(Human C-term) AP2723b
    Anti-WIF1 Antibody(Human C-term) at 1:1000 dilution + Mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q9Y5W5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41528 Da
Additional info
Gene ID 11197
Other Names Wnt inhibitory factor 1, WIF-1, WIF1
Target/Specificity This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 347-376 amino acids from the C-terminal region of human WIF1.
Dilution IHC-P-Leica~~1:500
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWIF1 Antibody(Human C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WIF1
Function Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Cellular Location Secreted.
Research Areas
Association between Wnt inhibitory factor 1 and receptor tyrosine kinase-like orphan receptor 2 protein expression and the clinical pathological significance in benign and malignant pancreatic lesions.
Author : Zhu D1,2,Yang Z3,Liu Z3,Zou Q4,Yuan Y4,Hu C1.
Oncol Lett. 2017 Apr;13(4):2244-2252. doi: 10.3892/ol.2017.5704. Epub 2017 Feb 8.
28454387

BACKGROUND

WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.

REFERENCES

Elston,M.S., Endocrinology 149 (3), 1235-1242 (2008)
Clement,G., Cancer Sci. 99 (1), 46-53 (2008)
Chan,S.L., Lab. Invest. 87 (7), 644-650 (2007)

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