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|Application ||WB, E|
|Other Accession||D3ZTD8, Q62217|
|Other Names||Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF|
|Target/Specificity||This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of mouse Sema5a.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||M Sema5a Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
Author : Lu TP, Tsai MH, Lee JM, Hsu CP, Chen PC, Lin CW, Shih JY, Yang PC, Hsiao CK, Lai LC, Chuang EY.
Cancer Epidemiol Biomarkers Prev. 2010 Oct;19(10):2590-7. doi: 10.1158/1055-9965.EPI-10-0332. Epub 2010 Aug 27.
Provided below are standard protocols that you may find useful for product applications.
Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development.
Bialecka,M., Neurosci. Lett. 399 (1-2), 121-123 (2006)
Melin,M., Neuropsychobiology 54 (1), 64-69 (2006)