注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   神经科学   >   M Sema5a Antibody (N-term)   

M Sema5a Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - M Sema5a  Antibody (N-term) AP2712a
    Anti-M Sema5a Antibody (N-term) at 1:1000 dilution + Mouse liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 文献引用 : 1
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q13591
Other Accession D3ZTD8, Q62217
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 9037
Other Names Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF
Target/Specificity This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of mouse Sema5a.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM Sema5a Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SEMA5A
Synonyms SEMAF
Function Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Identification of a novel biomarker, SEMA5A, for non-small cell lung carcinoma in nonsmoking women.
Author : Lu TP, Tsai MH, Lee JM, Hsu CP, Chen PC, Lin CW, Shih JY, Yang PC, Hsiao CK, Lai LC, Chuang EY.
Cancer Epidemiol Biomarkers Prev. 2010 Oct;19(10):2590-7. doi: 10.1158/1055-9965.EPI-10-0332. Epub 2010 Aug 27.
20802022

BACKGROUND

Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development.

REFERENCES

Bialecka,M., Neurosci. Lett. 399 (1-2), 121-123 (2006)
Melin,M., Neuropsychobiology 54 (1), 64-69 (2006)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价