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MeCP2 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MeCP2 Antibody  AP2545D
    All lanes : Anti-MeCP2(S423) Antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: A431 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - MeCP2 Antibody  AP2545D
    AP2545D staining MeCP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/200) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - MeCP2 Antibody  AP2545D
    AP2545D staining MeCP2 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - MeCP2 Antibody  AP2545D
    All lanes : Anti-MeCP2 Antibody at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: A431 whole cell lysates Lane 4: HepG2 whole cell lysates Lane 5: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession P51608
Other Accession Q9Z2D6, P51608, Q95LG8
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Target/Specificity This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from human MeCP2.
Dilution WB~~1:2000
IHC-P~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMeCP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

BACKGROUND

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

REFERENCES

dos Santos, J.M., et al., Neurosci. Lett. 379(1):13-16 (2005).
Ylisaukko-Oja, T., et al., Am J Med Genet A 132(2):121-124 (2005).
Schanen, C., et al., Am J Med Genet A 126(2):129-140 (2004).
Shibayama, A., et al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 128(1):50-53 (2004).
Fang, J.Y., et al., World J. Gastroenterol. 10(23):3394-3398 (2004).

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