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SPTLC1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SPTLC1 Antibody (C-term) AP2534b
    Western blot analysis of anti-SPTLC1 Antibody (C-term)(Cat.#AP2534b) in 293 cell line lysates (35ug/lane).SPTLC1(arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O15269
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52744 Da
Additional info
Gene ID 10558
Other Names Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1, SPTLC1, LCB1
Target/Specificity This SPTLC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-437 amino acids from the C-terminal region of human SPTLC1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPTLC1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPTLC1
Synonyms LCB1
Function Serine palmitoyltransferase (SPT) (PubMed:19416851). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core (PubMed:19416851). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed:19416851). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1- SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed:19416851). The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed:19416851). Required for adipocyte cell viability and metabolic homeostasis (By similarity).
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O35704}; Single-pass membrane protein {ECO:0000250|UniProtKB:O35704}
Tissue Location Widely expressed. Not detected in small intestine.
Research Areas
Serine palmitoyltransferase subunit 1 is present in the endoplasmic reticulum, nucleus and focal adhesions, and functions in cell morphology.
Author : Wei J, Yerokun T, Leipelt M, Haynes CA, Radhakrishna H, Momin A, Kelly S, Park H, Wang E, Carton JM, Uhlinger DJ, Merrill AH Jr.
Biochim Biophys Acta. 2009 Aug;1791(8):746-56. doi: 10.1016/j.bbalip.2009.03.016. Epub 2009 Apr 9.
19362163
Apoptotic sphingolipid signaling by ceramides in lung endothelial cells.
Author : Medler TR1, Petrusca DN, Lee PJ, Hubbard WC, Berdyshev EV, Skirball J, Kamocki K, Schuchman E, Tuder RM, Petrache I.
Am J Respir Cell Mol Biol. 2008 Jun;38(6):639-46. doi: 10.1165/rcmb.2007-0274OC. Epub 2008 Jan 10.
18192502

BACKGROUND

Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.

REFERENCES

Stachowitz, S., et al., J. Invest. Dermatol. 119(5):1048-1052 (2002).
Nicholson, G.A., et al., Am. J. Hum. Genet. 69(3):655-659 (2001).
Dawkins, J.L., et al., Nat. Genet. 27(3):309-312 (2001).
Bejaoui, K., et al., Nat. Genet. 27(3):261-262 (2001).
Perry, D.K., et al., J. Biol. Chem. 275(12):9078-9084 (2000).

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