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GPI8 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - GPI8 Antibody (N-term) AP2462a
    The anti-GPI8 Pab (Cat. #AP2462a) is used in Western blot to detect GPI8 in 293 cell lysate.
  • 14 - GPI8 Antibody (N-term) AP2462a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q92643
Other Accession Q4KRV1, Q9CXY9, Q3MHZ7
Reactivity Human
Predicted Bovine, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45252 Da
Additional info
Gene ID 10026
Other Names GPI-anchor transamidase, GPI transamidase, 3---, GPI8 homolog, hGPI8, Phosphatidylinositol-glycan biosynthesis class K protein, PIG-K, PIGK, GPI8
Target/Specificity This GPI8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-68 amino acids from the N-terminal region of human GPI8.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPI8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIGK
Synonyms GPI8
Function Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein
Research Areas
Profiling the expression pattern of GPI transamidase complex subunits in human cancer.
Author : Nagpal JK, Dasgupta S, Jadallah S, Chae YK, Ratovitski EA, Toubaji A, Netto GJ, Eagle T, Nissan A, Sidransky D, Trink B.
Mod Pathol. 2008 Aug;21(8):979-91. doi: 10.1038/modpathol.2008.76. Epub 2008 May 16.
18487995

BACKGROUND

GPI8 is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.

REFERENCES

Ohishi, K., et al., J. Biol. Chem. 278(16):13959-13967 (2003). Vainauskas, S., et al., J. Biol. Chem. 277(34):30535-30542 (2002). Ohishi, K., et al., EMBO J. 20(15):4088-4098 (2001). Meyer, U., et al., Biochemistry 39(12):3461-3471 (2000). Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 94(23):12580-12585 (1997).

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