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|Application ||IHC-P, WB, E|
|Other Names||Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER, RAGE|
|Target/Specificity||This RAGE (AGER) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-59 amino acids from the N-terminal region of human RAGE (AGER).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAGE (AGER) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 10: Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Endothelial cells.|
Author : Chen S1, Yin L1, Xu Z1, An FM1, Liu AR2, Wang Y1, Yao WB3, Gao XD4.
Neurosci Lett. 2015 Dec 8. pii: S0304-3940(15)30295-0. doi: 10.1016/j.neulet.2015.12.007. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
AGER is a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products.
Schlueter, C., et al., Biochim. Biophys. Acta 1630(1):1-6 (2003). Shanmugam, N., et al., J. Biol. Chem. 278(37):34834-34844 (2003). Kuniyasu, H., et al., Oncol. Rep. 10(2):445-448 (2003). Hsieh, H.L., et al., Biochem. Biophys. Res. Commun. 307(2):375-381 (2003). Rocken, C., et al., Am. J. Pathol. 162(4):1213-1220 (2003).