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HIST1H3A

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HIST1H3A AP22384a
    All lanes : Anti-HIST1H3A at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: C2C12 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P68431
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 15404 Da
Additional info
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Other Names Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1~31 amino acids from human.
Dilution WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H3A is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H3A
Synonyms H3FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.

BACKGROUND

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

REFERENCES

Zhong R.,et al.Nucleic Acids Res. 11:7409-7425(1983).
Marashi F.,et al.Biochem. Cell Biol. 64:277-289(1986).
Albig W.,et al.Genomics 10:940-948(1991).
Kardalinou E.,et al.J. Cell. Biochem. 52:375-383(1993).
Runge D.,et al.Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.

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