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MAP1LC3A antibody(N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 59 - MAP1LC3A antibody(N-term) AP22378a
    Immunohistochemical analysis of paraffin-embedded human heart tissue using AP22378a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - MAP1LC3A antibody(N-term) AP22378a
    Immunohistochemical analysis of paraffin-embedded human brain tissue using AP22378a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - MAP1LC3A antibody(N-term) AP22378a
    All lanes : Anti-MAP1LC3A antibody(N-term) at 1:2000 dilution Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - MAP1LC3A antibody(N-term) AP22378a
    All lanes : Anti-MAP1LC3A antibody(N-term) at 1:500 dilution Lane 1: Human brain tissue lysate Lane 2: U-251 MG whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q9H492
Other Accession Q2HJ23, Q91VR7, Q6XVN8, Q6NX90
Reactivity Human, Mouse, Rat
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 14272 Da
Additional info
Gene ID 84557
Other Names Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-40 amino acids from human.
Dilution IHC-P-Leica~~1:500
WB~~1:500
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP1LC3A antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP1LC3A
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).
Cellular Location Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome Note=LC3-II binds to the autophagic membranes
Tissue Location Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Research Areas

BACKGROUND

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

REFERENCES

He H.,et al.J. Biol. Chem. 278:29278-29287(2003).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Deloukas P.,et al.Nature 414:865-871(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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