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CDC25A (S124) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - CDC25A (S124) Antibody AP22373a
    Immunohistochemical analysis of AP22373a on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - CDC25A (S124) Antibody AP22373a
    All lanes : Anti-CDC25A(S124) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CDC25A (S124) Antibody AP22373a
    All lanes : Anti-CDC25A(S124) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P30304
Reactivity Human
Predicted Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 59087 Da
Additional info
Gene ID 993
Other Names M-phase inducer phosphatase 1, 3.1.3.48, Dual specificity phosphatase Cdc25A, CDC25A
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
Dilution IHC-P~~1:100
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC25A (S124) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25A
Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Research Areas

BACKGROUND

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

REFERENCES

Galaktionov K.I.,et al.Cell 67:1181-1194(1991).
Varmeh-Ziaie S.,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Wegener S.,et al.Eur. J. Cell Biol. 79:810-815(2000).
Falck J.,et al.Nature 410:842-847(2001).
Donzelli M.,et al.EMBO J. 21:4875-4884(2002).

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