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GPX7 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - GPX7 Antibody (Center) AP22347c
    Overlay histogram showing U-2 OS cells stained with AP22347c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22347c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - GPX7 Antibody (Center) AP22347c
    All lanes : Anti-GPX7 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: RPMI 8226 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q96SL4
Other Accession A6QLY2
Reactivity Human
Predicted Bovine, Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 20996 Da
Additional info
Gene ID 2882
Other Names Glutathione peroxidase 7, GPx-7, GSHPx-7, 1.11.1.9, CL683, GPX7, GPX6
Target/Specificity This GPX7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 73-107 amino acids from the Central region of human GPX7.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPX7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPX7
Synonyms GPX6
Function It protects esophageal epithelia from hydrogen peroxide- induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks.
Cellular Location Secreted.
Tissue Location Expressed in esophageal epithelial cells; expression is up-regulated after exposure to acidic bile acids
Research Areas

BACKGROUND

It protects esophageal epithelia from hydrogen peroxide- induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks.

REFERENCES

Gu S.,et al.Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Barrow I.K.-P.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.

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