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>   首页   >   产品   >   一抗   >   神经科学   >   KCNH1 Antibody (C-Term)   

KCNH1 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - KCNH1 Antibody (C-Term) AP22343b
    Overlay histogram showing Hela cells stained with AP22343b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22343b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - KCNH1 Antibody (C-Term) AP22343b
    All lanes : Anti-KCNH1 Antibody (C-Term) at 1:2000 dilution Lane 1: COLO 205 whole cell lysate Lane 2: Human brain lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 111 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession O95259
Reactivity Human
Predicted Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 111423 Da
Additional info
Gene ID 3756
Other Names Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1, EAG channel 1, h-eag, hEAG1, Voltage-gated potassium channel subunit Kv10.1, KCNH1, EAG, EAG1
Target/Specificity This KCNH1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 787-820 amino acids from the human region of human KCNH1.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKCNH1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KCNH1
Synonyms EAG, EAG1
Function Pore-forming (alpha) subunit of voltage-gated non- inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.
Cellular Location Cell membrane; Multi-pass membrane protein. Nucleus inner membrane; Multi-pass membrane protein. Note=Perinuclear KCNH1 is located to NPC-free islands
Tissue Location Highly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells
Research Areas

BACKGROUND

Pore-forming (alpha) subunit of voltage-gated non- inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.

REFERENCES

Occhiodoro T.,et al.FEBS Lett. 434:177-182(1998).
Pardo L.A.,et al.EMBO J. 18:5540-5547(1999).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Schoenherr R.,et al.EMBO J. 19:3263-3271(2000).

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