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BRAF Antiboday(C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - BRAF Antiboday(C-Term) AP22342b
    AP22342b staining BRAF in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - BRAF Antiboday(C-Term) AP22342b
    All lanes : Anti-BRAF Antiboday(C-Term) at 1:2000 dilution Lane 1: Braf-600V lysate Lane 2: Braf-600E lysate Lane 3: Raji whole cell lysate Lane 4: Hela whole cell lysate Lane 5: A549 whole cell lysate Lane 6: T47D whole cell lysate Lane 7: MDA-MB-453 whole cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P15056
Other Accession Q04982, P34908, P28028, P00531, A7E3S4, P05625, P04049, Q99N57, Q5R5M7, P11345, P00532, P27966, P10533
Reactivity Human
Predicted Bovine, Chicken, Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 84437 Da
Additional info
Gene ID 673
Other Names Serine/threonine-protein kinase B-raf, 2.7.11.1, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from human.
Dilution IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRAF Antiboday(C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRAF
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas

BACKGROUND

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

REFERENCES

Stephens R.M.,et al.Mol. Cell. Biol. 12:3733-3742(1992).
Albert S.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
Hillier L.W.,et al.Nature 424:157-164(2003).
Eychene A.,et al.Oncogene 7:1657-1660(1992).

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