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>   首页   >   产品   >   一抗   >   神经科学   >   APLP1 Antibody (C-Term)   

APLP1 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - APLP1 Antibody (C-Term) AP22338b
    Overlay histogram showing U-87 MG cells stained with AP22338b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22338b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - APLP1 Antibody (C-Term) AP22338b
    All lanes : Anti-APLP1 Antibody (C-Term) at 1:2000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P51693
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 72176 Da
Additional info
Gene ID 333
Other Names Amyloid-like protein 1, APLP, APLP-1, C30, APLP1
Target/Specificity This APLP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 505-539 amino acids from the human region of human APLP1.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPLP1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APLP1
Function May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD)
Research Areas

BACKGROUND

May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.

REFERENCES

Paliga K.,et al.Eur. J. Biochem. 250:354-363(1997).
Lenkkeri U.,et al.Hum. Genet. 102:192-196(1998).
Grimwood J.,et al.Nature 428:529-535(2004).
Kim T.-W.,et al.Brain Res. Mol. Brain Res. 32:36-44(1995).
Bush A.I.,et al.J. Biol. Chem. 269:26618-26621(1994).

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