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beta Tubulin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - beta Tubulin Antibody AP22332a
    Anti-beta Tubulin Antibody at 1:2000 dilution + Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P99024
Other Accession Q17299, P12456, P09203, Q24560, Q9YHC3, Q27U48, Q25009, O17449, P36221, Q13885, Q4R5B3, Q7TMM9, P85108, Q6B856, Q9BVA1, Q9CWF2, Q3KRE8, P32882, P83130, P61858, P61857, Q9NFZ6, P13602, Q2T9S0, P09206, P08841, Q13509, Q60HC2, Q9ERD7, Q4QRB4, Q3ZBU7, P04350, Q4R4X8, Q9D6F9, Q3MHM5, P68371, P68
Reactivity Mouse
Predicted Human, Mouse, Rat, Pig, Chicken, Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 22154
Other Names Tubulin beta-5 chain, Tubb5
Target/Specificity This beta Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 298-328 amino acids from the mouse region of mouse beta Tubulin.
Dilution WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsbeta Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tubb5
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Expressed in embryonic brain, including throughout the developing cortex and in the subventricular zone. Also found in radial glial cells, intermediate progenitors, migrating neurons and postmitotic neurons.
Research Areas

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

REFERENCES

Wang D.,et al.J. Cell Biol. 103:1903-1910(1986).
Carninci P.,et al.Science 309:1559-1563(2005).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.
Lewis S.A.,et al.J. Cell Biol. 101:852-861(1985).

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