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SCN1A Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - SCN1A Antibody (Center) AP22326c
    Overlay histogram showing U-87 MG cells stained with AP22326c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22326c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - SCN1A Antibody (Center) AP22326c
    Anti-SCN1A Antibody (Center) at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 229 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P35498
Other Accession P04774
Reactivity Human
Predicted Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 228972 Da
Additional info
Gene ID 6323
Other Names Sodium channel protein type 1 subunit alpha, Sodium channel protein brain I subunit alpha, Sodium channel protein type I subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.1, SCN1A, NAC1, SCN1
Target/Specificity This SCN1A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 481-515 amino acids from the Central region of human SCN1A.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCN1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SCN1A
Synonyms NAC1, SCN1
Function Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
Cellular Location Membrane; Multi-pass membrane protein.
Research Areas

BACKGROUND

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.

REFERENCES

Escayg A.,et al.Nat. Genet. 24:343-345(2000).
Jeong S.-Y.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Sugawara T.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Ouchida M.,et al.Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).

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