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MYL1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - MYL1 Antibody (Center) AP22325c
    AP22325c staining MYL1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 4 - MYL1 Antibody (Center) AP22325c
    Overlay histogram showing Hela cells stained with AP22325c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22325c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - MYL1 Antibody (Center) AP22325c
    All lanes : Anti-MYL1 Antibody (Center) at 1:2000 dilution Lane 1: Human heart lysate Lane 2: Human skeletal muslce lysate Lane 3: Mouse skeletal muscle lysate Lane 4: Rat skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P05976
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 21145 Da
Additional info
Gene ID 4632
Other Names Myosin light chain 1/3, skeletal muscle isoform, MLC1/MLC3, MLC1F/MLC3F, Myosin light chain alkali 1/2, Myosin light chain A1/A2, MYL1
Target/Specificity This MYL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-135 amino acids from the Central region of human MYL1.
Dilution IHC-P~~1:25
FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYL1
Function Regulatory light chain of myosin. Does not bind calcium.
Research Areas

BACKGROUND

Regulatory light chain of myosin. Does not bind calcium.

REFERENCES

Seidel U.,et al.Nucleic Acids Res. 15:4989-4989(1987).
Seidel U.,et al.Gene 66:135-146(1988).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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