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CCL26 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

Overlay histogram showing K562 cells stained with AP22319b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22319b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-CCL26 Antibody (C-Term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: K562 whole cell lysate Lane 4: NCI-H460 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, WB, E
Primary Accession	Q9Y258
Reactivity	Human
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit Ig
Calculated MW	10648 Da

Additional info
Gene ID	10344
Other Names	C-C motif chemokine 26, CC chemokine IMAC, Eotaxin-3, Macrophage inflammatory protein 4-alpha, MIP-4-alpha, Small-inducible cytokine A26, Thymic stroma chemokine-1, TSC-1, CCL26, SCYA26
Target/Specificity	This CCL26 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 58-92 amino acids from the human region of human CCL26.
Dilution	FC~~1:25 WB~~1:2000
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CCL26 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CCL26
Synonyms	SCYA26
Function	Chemotactic for eosinophils and basophils. Binds to CCR3.
Cellular Location	Secreted.
Tissue Location	Ubiquitously expressed at low levels in various tissues including heart and ovary

Research Areas

BACKGROUND

Chemotactic for eosinophils and basophils. Binds to CCR3.

REFERENCES

Guo R.F.,et al.Genomics 58:313-317(1999).
Shinkai A.,et al.J. Immunol. 163:1602-1610(1999).
Kitaura M.,et al.J. Biol. Chem. 274:27975-27980(1999).
Wang Z.,et al.Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
Berg E.L.,et al.Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.

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¥ 2,310.00

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Cat# AP22319b

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CCL26 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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