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>   首页   >   产品   >   一抗   >   细胞生物学   >   NAT10 Antibody (N-Term)   

NAT10 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - NAT10 Antibody (N-Term) AP22316a
    Overlay histogram showing Hela cells stained with AP22316a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22316a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - NAT10 Antibody (N-Term) AP22316a
    All lanes : Anti-NAT10 Antibody (N-Term) at 1:2000 dilution Lane 1: Human lung lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 116 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q9H0A0
Other Accession Q8K224
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 115730 Da
Additional info
Gene ID 55226
Other Names N-acetyltransferase 10, 2.3.1.-, NAT10, ALP, KIAA1709
Target/Specificity This NAT10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 146-178 amino acids from the mouse region of mouse NAT10.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNAT10 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NAT10
Synonyms ALP, KIAA1709
Function Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division.
Cellular Location Nucleus, nucleolus. Note=Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase
Research Areas

BACKGROUND

Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division.

REFERENCES

Nagase T.,et al.DNA Res. 7:347-355(2000).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).
Lv J.,et al.Biochem. Biophys. Res. Commun. 311:506-513(2003).

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