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CLCN1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - CLCN1 Antibody (N-Term) AP22311a
    Overlay histogram showing Hela cells stained with AP22311a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22311a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - CLCN1 Antibody (N-Term) AP22311a
    All lanes : Anti-CLCN1 Antibody (N-Term) at 1:2000 dilution Lane 1: Human skeletal muslce lysate Lane 2: Mouse skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P35523
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 108626 Da
Additional info
Gene ID 1180
Other Names Chloride channel protein 1, ClC-1, Chloride channel protein, skeletal muscle, CLCN1, CLC1
Target/Specificity This CLCN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-66 amino acids from the human region of human CLCN1.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLCN1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLCN1
Synonyms CLC1
Function Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.
Cellular Location Membrane; Multi-pass membrane protein.
Tissue Location Predominantly expressed in skeletal muscles.
Research Areas

BACKGROUND

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.

REFERENCES

Steinmeyer K.,et al.EMBO J. 13:737-743(1994).
Scherer S.W.,et al.Science 300:767-772(2003).
Koch M.C.,et al.Science 257:797-800(1992).
George A.L. Jr.,et al.Nat. Genet. 3:305-310(1993).
Lorenz C.,et al.Hum. Mol. Genet. 3:941-946(1994).

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