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TCIRG1 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - TCIRG1 Antibody (C-Term) AP22308b
    Overlay histogram showing U-2 OS cells stained with AP22308b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22308b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - TCIRG1 Antibody (C-Term) AP22308b
    Anti-TCIRG1 Antibody (C-Term) at 1:2000 dilution + EL4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q13488
Reactivity Mouse
Predicted Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 92968 Da
Additional info
Gene ID 10312
Other Names V-type proton ATPase 116 kDa subunit a isoform 3, V-ATPase 116 kDa isoform a3, Osteoclastic proton pump 116 kDa subunit, OC-116 kDa, OC116, T-cell immune regulator 1, T-cell immune response cDNA7 protein, TIRC7, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3, TCIRG1, ATP6N1C, ATP6V0A3
Target/Specificity This TCIRG1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 668-702 amino acids from the human region of human TCIRG1.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCIRG1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCIRG1
Synonyms ATP6N1C, ATP6V0A3
Function Part of the proton channel of V-ATPases (By similarity). Seems to be directly involved in T-cell activation.
Cellular Location Membrane; Multi-pass membrane protein
Tissue Location Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus
Research Areas

BACKGROUND

Part of the proton channel of V-ATPases (By similarity). Seems to be directly involved in T-cell activation.

REFERENCES

Li Y.P.,et al.Biochem. Biophys. Res. Commun. 218:813-821(1996).
Utku N.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Heinemann T.,et al.Genomics 57:398-406(1999).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Sobacchi C.,et al.Hum. Mol. Genet. 10:1767-1773(2001).

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