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BCL2L10 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - BCL2L10 Antibody (Center) AP22305c
    Overlay histogram showing A549 cells stained with AP22305c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22305c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 3 - BCL2L10 Antibody (Center) AP22305c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling BCL2L10 with AP22305c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
  • 1 - BCL2L10 Antibody (Center) AP22305c
    All lanes : Anti-BCL2L10 Antibody (Center) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: Human liver lysate Lane 3: Human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IF, WB, E
Primary Accession Q9HD36
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 21973 Da
Additional info
Gene ID 10017
Other Names Bcl-2-like protein 10, Bcl2-L-10, Anti-apoptotic protein NrH, Apoptosis regulator Bcl-B, BCL2L10, BCLB
Target/Specificity This BCL2L10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 89-123 amino acids from the Central region of human BCL2L10.
Dilution FC~~1:25
IF~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCL2L10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2L10
Synonyms BCLB
Function Promotes cell survival. Suppresses apoptosis induced by BAX but not BAK.
Cellular Location Mitochondrion. Nucleus membrane
Tissue Location Widely expressed in adult tissues. Preferentially expressed in lung, liver and kidney
Research Areas

BACKGROUND

Promotes cell survival. Suppresses apoptosis induced by BAX but not BAK.

REFERENCES

Zhang H.,et al.Hum. Mol. Genet. 10:2329-2339(2001).
Ke N.,et al.J. Biol. Chem. 276:12481-12484(2001).
Aouacheria A.,et al.Oncogene 20:5846-5855(2001).
Zody M.C.,et al.Nature 440:671-675(2006).

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