注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   TGFA Antibody (C-Term)   

TGFA Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - TGFA Antibody (C-Term) AP22303b
    Overlay histogram showing A431 cells stained with AP22303b(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - TGFA Antibody (C-Term) AP22303b
    All lanes : Anti-TGFA Antibody (C-Term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HACAT whole cell lysate Lane 3: RPMI 8226 whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P01135
Other Accession P48030, Q06922, P01134
Reactivity Human
Predicted Mouse, Pig, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Additional info
Other Names Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA
Target/Specificity This TGFA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 118-150 amino acids from the human region of human TGFA.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTGFA Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGFA
Function TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Cellular Location Transforming growth factor alpha: Secreted, extracellular space
Tissue Location Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines EMBL; K03222; AAA61159.1; -; mRNA EMBL; M31172; AAA61157.1; -; mRNA EMBL; X70340; CAA49806.1; -; mRNA EMBL; AF123243; AAF13491.1; -; Genomic_DNA EMBL; AF123238; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123239; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123240; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123241; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123242; AAF13491.1; JOINED; Genomic_DNA EMBL; AY325886; AAP97822.2; -; Genomic_DNA EMBL; AY325885; AAP97822.2; JOINED; Genomic_DNA EMBL; AY326405; AAP97822.2; JOINED; Genomic_DNA EMBL; AY327131; AAP97822.2; JOINED; Genomic_DNA EMBL; AY327132; AAP97822.2; JOINED; Genomic_DNA EMBL; AY329368; AAP97822.2; JOINED; Genomic_DNA EMBL; AF149096; AAF05089.1; -; mRNA EMBL; AF149097; AAF05090.1; -; mRNA EMBL; AF149098; AAF05091.1; -; mRNA EMBL; BT006833; AAP35479.1; -; mRNA EMBL; AK290151; BAF82840.1; -; mRNA EMBL; AC005234; AAY14793.1; -; Genomic_DNA EMBL; AC017084; AAY14705.1; -; Genomic_DNA EMBL; CH471053; EAW99810.1; -; Genomic_DNA EMBL; CH471053; EAW99812.1; -; Genomic_DNA EMBL; BC005308; AAH05308.1; -; mRNA EMBL; M22440; AAA52530.1; -; Genomic_DNA EMBL; AF075584; AAD12238.1; -; Genomic_DNA EMBL; AF075583; AAD12238.1; JOINED; Genomic_DNA CCDS; CCDS1905.1; -. [P01135-1] CCDS; CCDS46316.1; -. [P01135-2] PIR; JN0876; WFHU1 RefSeq; NP_001093161.1; NM_001099691.2. [P01135-2] RefSeq; NP_001295087.1; NM_001308158.1 RefSeq; NP_001295088.1; NM_001308159.1 RefSeq; NP_003227.1; NM_003236.3. [P01135-1] UniGene; Hs.170009; - UniGene; Hs.628298; - PDB; 1GK5; NMR; -; A=83-89 PDB; 1MOX; X-ray; 2.50 A; C/D=40-89 PDB; 1YUF; NMR; -; A=40-89 PDB; 1YUG; NMR; -; A=40-89 PDB; 2TGF; NMR; -; A=40-89 PDB; 3E50; X-ray; 2.30 A; C/D=40-89 PDB; 3TGF; NMR; -; A=40-89 PDB; 4TGF; NMR; -; A=40-89 PDB; 5KN5; X-ray; 2.80 A; C/F=49-88 PDBsum; 1GK5; - PDBsum; 1MOX; - PDBsum; 1YUF; - PDBsum; 1YUG; - PDBsum; 2TGF; - PDBsum; 3E50; - PDBsum; 3TGF; - PDBsum; 4TGF; - PDBsum; 5KN5; - ProteinModelPortal; P01135; - SMR; P01135; - BioGrid; 112897; 68 DIP; DIP-5765N; - IntAct; P01135; 10 MINT; P01135; - STRING; 9606.ENSP00000295400; - SwissPalm; P01135; - BioMuta; TGFA; - DMDM; 135689; - jPOST; P01135; - PaxDb; P01135; - PeptideAtlas; P01135; - PRIDE; P01135; - ProteomicsDB; 51332; - ProteomicsDB; 51333; -. [P01135-2] ProteomicsDB; 51334; -. [P01135-3] ProteomicsDB; 51335; -. [P01135-4] ProteomicsDB; 51336; -. [P01135-5] DNASU; 7039; - Ensembl; ENST00000295400; ENSP00000295400; ENSG00000163235. [P01135-1] Ensembl; ENST00000418333; ENSP00000404099; ENSG00000163235. [P01135-2] Ensembl; ENST00000445399; ENSP00000387493; ENSG00000163235. [P01135-3] GeneID; 7039; - KEGG; hsa:7039; - UCSC; uc002sgs.4; human. [P01135-1] CTD; 7039; - DisGeNET; 7039; - EuPathDB; HostDB:ENSG00000163235.15; - GeneCards; TGFA; - HGNC; HGNC:11765; TGFA HPA; HPA042297; - MalaCards; TGFA; - MIM; 190170; gene neXtProt; NX_P01135; - OpenTargets; ENSG00000163235; - Orphanet; 2227; NON RARE IN EUROPE: Hypodontia Orphanet; 99798; Oligodontia PharmGKB; PA36480; - eggNOG; ENOG410IVW5; Eukaryota eggNOG; ENOG4111Z4I; LUCA GeneTree; ENSGT00940000160058; - HOGENOM; HOG000013036; - HOVERGEN; HBG000330; - InParanoid; P01135; - KO; K08774; - OMA; CQALICR; - OrthoDB; 1314811at2759; - PhylomeDB; P01135; - TreeFam; TF332938; - Reactome; R-HSA-1257604; PIP3 activates AKT signaling Reactome; R-HSA-177929; Signaling by EGFR Reactome; R-HSA-179812; GRB2 events in EGFR signaling Reactome; R-HSA-180292; GAB1 signalosome Reactome; R-HSA-180336; SHC1 events in EGFR signaling Reactome; R-HSA-182971; EGFR downregulation Reactome; R-HSA-204005; COPII-mediated vesicle transport Reactome; R-HSA-212718; EGFR interacts with phospholipase C-gamma Reactome; R-HSA-2219530; Constitutive Signaling by Aberrant PI3K in Cancer Reactome; R-HSA-5638303; Inhibition of Signaling by Overexpressed EGFR Reactome; R-HSA-5673001; RAF/MAP kinase cascade Reactome; R-HSA-5694530; Cargo concentration in the ER Reactome; R-HSA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling Reactome; R-HSA-8856825; Cargo recognition for clathrin-mediated endocytosis Reactome; R-HSA-8856828; Clathrin-mediated endocytosis Reactome; R-HSA-8866910; TFAP2 (AP-2) family regulates transcription of growth factors and their receptors Reactome; R-HSA-9018519; Estrogen-dependent gene expression SignaLink; P01135; - SIGNOR; P01135; - ChiTaRS; TGFA; human EvolutionaryTrace; P01135; - GeneWiki; TGF_alpha; - GenomeRNAi; 7039; - PMAP-CutDB; P01135; - PRO; PR:P01135; - Proteomes; UP000005640; Chromosome 2 Bgee; ENSG00000163235; Expressed in 192 organ(s), highest expression level in pigmented layer of retina ExpressionAtlas; P01135; baseline and differential Genevisible; P01135; HS GO; GO:0016323; C:basolateral plasma membrane; IDA:BHF-UCL GO; GO:0009986; C:cell surface; IDA:BHF-UCL GO; GO:0031410; C:cytoplasmic vesicle; IDA:BHF-UCL GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; TAS:Reactome GO; GO:0012507; C:ER to Golgi transport vesicle membrane; TAS:Reactome GO; GO:0005615; C:extracellular space; IDA:BHF-UCL GO; GO:0000139; C:Golgi membrane; IEA:GOC GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL GO; GO:0005886; C:plasma membrane; NAS:ProtInc GO; GO:0005154; F:epidermal growth factor receptor binding; IDA:UniProtKB GO; GO:0008083; F:growth factor activity; IDA:HGNC GO; GO:0000187; P:activation of MAPK activity; IDA:HGNC GO; GO:0008283; P:cell population proliferation; TAS:ProtInc GO; GO:0048208; P:COPII vesicle coating; TAS:Reactome GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; TAS:Reactome GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IBA:GO_Central GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central GO; GO:0045741; P:positive regulation of epidermal growth factor-activated receptor activity; IDA:HGNC GO; GO:0050679; P:positive regulation of epithelial cell proliferation; IDA:HGNC GO; GO:0045840; P:positive regulation of mitotic nuclear division; IDA:HGNC GO; GO:0006357; P:regulation of transcription by RNA polymerase II; TAS:Reactome InterPro; IPR013032; EGF-like_CS InterPro; IPR000742; EGF-like_dom InterPro; IPR015497; EGF_rcpt_ligand PANTHER; PTHR10740; PTHR10740; 1 PROSITE; PS00022; EGF_1; 1 PROSITE; PS01186; EGF_2; 1 PROSITE; PS50026; EGF_3; 1 1: Evidence at protein level; 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Disulfide bond; EGF-like domain; Glycoprotein; Growth factor; Lipoprotein; Membrane; Mitogen; Palmitate; Polymorphism; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix SIGNAL 1 23 CHAIN 24 160 Protransforming growth factor alpha /FTId=PRO_0000302744 PROPEP 24 39 Removed in mature form /FTId=PRO_0000007752 CHAIN 40 89 Transforming growth factor alpha /FTId=PRO_0000007753 PROPEP 90 160 Removed in mature form /FTId=PRO_0000007754 TOPO_DOM 24 98 Extracellular. TRANSMEM 99 124 Helical. TOPO_DOM 125 160 Cytoplasmic. DOMAIN 43 83 EGF-like. {ECO:0000255|PROSITE- ProRule:PRU00076} LIPID 153 153 S-palmitoyl cysteine LIPID 154 154 S-palmitoyl cysteine CARBOHYD 25 25 N-linked (GlcNAc...) asparagine DISULFID 47 60 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:1632509} DISULFID 55 71 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:1632509} DISULFID 73 82 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:1632509} VAR_SEQ 32 32 Missing (in isoform 2, isoform 3 and isoform 5). {ECO:0000303|PubMed:10523832, ECO:0000303|PubMed:15489334, ECO:0000303|Ref.7} /FTId=VSP_038369 VAR_SEQ 159 160 VV -> ATLG (in isoform 3) /FTId=VSP_038370 VAR_SEQ 159 160 VV -> GCRLY (in isoform 4 and isoform 5) /FTId=VSP_038371 VARIANT 109 109 V -> M (in dbSNP:rs11466259) /FTId=VAR_024271 CONFLICT 58 58 G -> A (in Ref. 2; AAA61157) CONFLICT 65 65 Q -> H (in Ref. 2; AAA61157) CONFLICT 159 159 V -> L (in Ref. 2; AAA61157) STRAND 43 45 {ECO:0000244|PDB:1MOX} HELIX 51 54 {ECO:0000244|PDB:1YUF} STRAND 58 63 {ECO:0000244|PDB:1MOX} TURN 64 67 {ECO:0000244|PDB:1MOX} STRAND 68 73 {ECO:0000244|PDB:1MOX} STRAND 77 79 {ECO:0000244|PDB:1MOX} STRAND 84 86 {ECO:0000244|PDB:5KN5} SEQUENCE 160 AA; 17006 MW; D692184F9353DE47 CRC64; MVPSAGQLAL FALGIVLAAC QALENSTSPL SADPPVAAAV VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLAV VAASQKKQAI TALVVVSIVA LAVLIITCVL IHCCQVRKHC EWCRALICRH EKPSALLKGR TACCHSETVV
Research Areas

BACKGROUND

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

REFERENCES

Derynck R.,et al.Cell 38:287-297(1984).
Jakowlew S.B.,et al.Mol. Endocrinol. 2:1056-1063(1988).
Qian J.F.,et al.Gene 132:291-296(1993).
Qian J.F.,et al.Am. J. Hum. Genet. 53:168-175(1993).
Machida J.,et al.Genomics 61:237-242(1999).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价