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ATP1A4 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - ATP1A4 Antibody (N-Term) AP22300a
    Overlay histogram showing HeLa cells stained with AP22300a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22300a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ATP1A4 Antibody (N-Term) AP22300a
    Anti-ATP1A4 Antibody (N-Term) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 114 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q13733
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 114166 Da
Additional info
Gene ID 480
Other Names Sodium/potassium-transporting ATPase subunit alpha-4, Na(+)/K(+) ATPase alpha-4 subunit, 3.6.3.9, Sodium pump subunit alpha-4, ATP1A4, ATP1AL2
Target/Specificity This ATP1A4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12-46 amino acids from the human region of human ATP1A4.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP1A4 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP1A4
Synonyms ATP1AL2
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.
Cellular Location Cell membrane; Multi-pass membrane protein. Note=In mature sperm, located in the principle piece of the sperm flagellum
Tissue Location Specifically expressed in testis. Found in very low levels in skeletal muscle. Expressed in mature sperm (at protein level)
Research Areas

BACKGROUND

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.

REFERENCES

Hlivko J.T.,et al.Mol. Reprod. Dev. 73:101-115(2006).
Gregory S.G.,et al.Nature 441:315-321(2006).
Keryanov S.,et al.Gene 292:151-166(2002).
Shamraj O.I.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:12952-12956(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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