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GYG1 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - GYG1 Antibody (C-Term) AP22264b
    Overlay histogram showing HepG2 cells stained with AP22264b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22264b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - GYG1 Antibody (C-Term) AP22264b
    All lanes : Anti-GYG1 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse heart lysate Lane 4: Mouse skeletal muscle lysate Lane 5: Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P46976
Other Accession Q9R062, O08730
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 39384 Da
Additional info
Gene ID 2992
Other Names Glycogenin-1, GN-1, GN1, 2.4.1.186, GYG1, GYG
Target/Specificity This GYG1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 314-347 amino acids from human GYG1.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGYG1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GYG1
Synonyms GYG
Function Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.
Research Areas

BACKGROUND

Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.

REFERENCES

Barbetti F.,et al.Biochem. Biophys. Res. Commun. 220:72-77(1996).
Lomako J.,et al.Genomics 33:519-522(1996).
Leffers H.,et al.Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
van Maanen M.-H.,et al.Gene 234:217-226(1999).
Zhai L.,et al.Gene 242:229-235(2000).

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