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PSMD3 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - PSMD3 Antibody (C-Term) AP22262b
    Overlay histogram showing HeLa cells stained with AP22262b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22262b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - PSMD3 Antibody (C-Term) AP22262b
    All lanes : Anti-PSMD3 Antibody (C-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession O43242
Other Accession Q2KJ46, P14685
Reactivity Human, Mouse
Predicted Bovine, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 60978 Da
Additional info
Gene ID 5709
Other Names 26S proteasome non-ATPase regulatory subunit 3, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, Proteasome subunit p58, PSMD3
Target/Specificity This PSMD3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 482-515 amino acids from human PSMD3.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMD3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMD3
Function Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Research Areas

BACKGROUND

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

REFERENCES

Kominami K.,et al.Mol. Biol. Cell 8:171-187(1997).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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