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ST8SIA1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ST8SIA1 Antibody (N-Term) AP22258a
    All lanes : Anti-ST8SIA1 Antibody (N-Term) at 1:1000-1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: A375 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q92185
Other Accession P61642
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 40519 Da
Additional info
Gene ID 6489
Other Names Alpha-N-acetylneuraminide alpha-2, 8-sialyltransferase, 2.4.99.8, Alpha-2, 8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8A
Target/Specificity This ST8SIA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 53-84 amino acids from human ST8SIA1.
Dilution WB~~1:1000-1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsST8SIA1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ST8SIA1
Synonyms SIAT8, SIAT8A
Function Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Strongly expressed in melanoma cell lines, adult and fetal brain and to a lesser extent in adult and fetal lung.
Research Areas

BACKGROUND

Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.

REFERENCES

Sasaki K.,et al.J. Biol. Chem. 269:15950-15956(1994).
Nara K.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:7952-7956(1994).
Haraguchi M.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:10455-10459(1994).
Nakayama J.,et al.J. Biol. Chem. 271:3684-3691(1996).
Rimoldi S.,et al.Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.

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