注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   信号转导   >   SYNE2 Antibody (N-Term)   

SYNE2 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - SYNE2 Antibody (N-Term) AP22254a
    AP22254a staining SYNE2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - SYNE2 Antibody (N-Term) AP22254a
    Anti-SYNE2 Antibody (N-Term) at 1:1000 dilution + Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 796 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q8WXH0
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 796442 Da
Additional info
Gene ID 23224
Other Names Nesprin-2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Protein NUANCE, Synaptic nuclear envelope protein 2, Syne-2, SYNE2, KIAA1011, NUA
Target/Specificity This SYNE2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1843-1877 amino acids from human SYNE2.
Dilution IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSYNE2 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SYNE2
Synonyms KIAA1011, NUA
Function Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein- containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for centrosome migration to the apical cell surface during early ciliogenesis.
Cellular Location Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side. Sarcoplasmic reticulum membrane; Single-pass type IV membrane protein. Cell membrane; Single-pass membrane protein Cytoplasm, cytoskeleton. Mitochondrion. Nucleus, nucleoplasm Note=Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell membrane. The largest part of the outer nuclear membrane- associated protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Shorter solubles isoforms can be found in the cytoplasm and within the nucleus
Tissue Location Widely expressed, with higher level in kidney, adult and fetal liver, stomach and placenta. Weakly expressed in skeletal muscle and brain. Isoform 5 is highly expressed in pancreas, skeletal muscle and heart
Research Areas

BACKGROUND

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein- containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for centrosome migration to the apical cell surface during early ciliogenesis.

REFERENCES

Zhen Y.-Y.,et al.J. Cell Sci. 115:3207-3222(2002).
Zhang Q.,et al.J. Cell Sci. 114:4485-4498(2001).
Zhang Q.,et al.Genomics 80:473-481(2002).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Heilig R.,et al.Nature 421:601-607(2003).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价