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TOMM40 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - TOMM40 Antibody (N-Term) AP22253a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling TOMM40 with AP22253a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 4 - TOMM40 Antibody (N-Term) AP22253a
    Overlay histogram showing HeLa cells stained with AP22253a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22253a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - TOMM40 Antibody (N-Term) AP22253a
    All lanes : Anti-TOMM40 Antibody (N-Term) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: Hut78 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: A431 whole cell lysate Lane 7: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, WB, E
Primary Accession O96008
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 37893 Da
Additional info
Gene ID 10452
Other Names Mitochondrial import receptor subunit TOM40 homolog, Protein Haymaker, Translocase of outer membrane 40 kDa subunit homolog, p38.5, TOMM40, C19orf1, PEREC1, TOM40
Target/Specificity This TOMM40 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from human TOMM40.
Dilution IF~~1:25
FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTOMM40 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TOMM40
Synonyms C19orf1, PEREC1, TOM40
Function Channel-forming protein essential for import of protein precursors into mitochondria.
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein
Research Areas

BACKGROUND

Channel-forming protein essential for import of protein precursors into mitochondria.

REFERENCES

Freitas E.M.,et al.DNA Seq. 9:89-100(1998).
Yoshiura K.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
Das B.,et al.Int. J. Cancer 94:800-806(2001).
Lubec G.,et al.Submitted (MAR-2007) to UniProtKB.
Johnston A.J.,et al.J. Biol. Chem. 277:42197-42204(2002).

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