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FAM125A Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 3 - FAM125A Antibody (N-Term) AP22249a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling FAM125A with AP22249a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 1 - FAM125A Antibody (N-Term) AP22249a
    Anti-FAM125A Antibody (N-Term) at 1:2000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession Q96EY5
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 28783 Da
Additional info
Gene ID 93343
Other Names Multivesicular body subunit 12A, CIN85/CD2AP family-binding protein, ESCRT-I complex subunit MVB12A, Protein FAM125A, MVB12A, CFBP, FAM125A
Target/Specificity This FAM125A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-50 amino acids from human FAM125A.
Dilution IF~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAM125A Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MVB12A
Synonyms CFBP, FAM125A
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization and down- regulation of EGF receptor.
Cellular Location Cytoplasm. Nucleus. Endosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Late endosome membrane; Peripheral membrane protein. Note=Colocalizes with F-actin. Some fraction may be nuclear
Tissue Location Ubiquitously expressed except in skeletal muscle.
Research Areas

BACKGROUND

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization and down- regulation of EGF receptor.

REFERENCES

Konishi H.,et al.J. Biol. Chem. 281:28919-28931(2006).
Beausoleil S.A.,et al.Nat. Biotechnol. 24:1285-1292(2006).
Morita E.,et al.Cell Host Microbe 2:41-53(2007).
Morita E.,et al.EMBO J. 26:4215-4227(2007).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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