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TMEM106B Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - TMEM106B Antibody (C-Term) AP22247b
    Overlay histogram showing Hela cells stained with AP22247b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22247b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - TMEM106B Antibody (C-Term) AP22247b
    Anti-TMEM106B Antibody (C-Term) at 1:2000 dilution + Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q9NUM4
Other Accession Q3ZC25, Q80X71, Q6AYA5
Reactivity Human, Mouse
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 31127 Da
Additional info
Gene ID 54664
Other Names Transmembrane protein 106B, TMEM106B
Target/Specificity This TMEM106B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 218-252 amino acids from human TMEM106B.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTMEM106B Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TMEM106B
Function Involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking via its interaction with MAP6. May act by inhibiting retrograde transport of lysosomes along dendrites. Required for dendrite branching.
Cellular Location Late endosome membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein
Tissue Location Expressed in frontal cortex.
Research Areas

BACKGROUND

Involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking via its interaction with MAP6. May act by inhibiting retrograde transport of lysosomes along dendrites. Required for dendrite branching.

REFERENCES

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).

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