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THBD Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - THBD Antibody (C-Term) AP22243b
    Overlay histogram showing A549 cells stained with AP22243b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22243b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - THBD Antibody (C-Term) AP22243b
    Anti-THBD Antibody (C-Term) at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P07204
Other Accession Q71U07
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 60329 Da
Additional info
Gene ID 7056
Other Names Thrombomodulin, TM, Fetomodulin, CD141, THBD, THRM
Target/Specificity This THBD antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 537-571 amino acids from human THBD.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTHBD Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name THBD
Synonyms THRM
Function Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Endothelial cells are unique in synthesizing thrombomodulin
Research Areas

BACKGROUND

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

REFERENCES

Suzuki K.,et al.EMBO J. 6:1891-1897(1987).
Wen D.,et al.Biochemistry 26:4350-4357(1987).
Jackman R.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:6425-6429(1987).
Shirai T.,et al.J. Biochem. 103:281-285(1988).
Deloukas P.,et al.Nature 414:865-871(2001).

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