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PGAP1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - PGAP1 Antibody (N-Term) AP22207a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PGAP1 with AP22207a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 4 - PGAP1 Antibody (N-Term) AP22207a
    Overlay histogram showing Hela cells stained with AP22207a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22207a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - PGAP1 Antibody (N-Term) AP22207a
    All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lane 4: mouse kidney lysate Lane 5: mouse testis lysate Lane 6: rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, WB, E
Primary Accession Q75T13
Other Accession Q3UUQ7, Q765A7
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 105383 Da
Additional info
Gene ID 80055
Other Names GPI inositol-deacylase, 3.1.-.-, Post-GPI attachment to proteins factor 1, hPGAP1, PGAP1
Target/Specificity This PGAP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 90-122 amino acids from human PGAP1.
Dilution IF~~1:25
FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGAP1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGAP1
Function Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein

BACKGROUND

Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity).

REFERENCES

Tanaka S.,et al.J. Biol. Chem. 279:14256-14263(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Hillier L.W.,et al.Nature 434:724-731(2005).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).

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