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OVGP1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - OVGP1 Antibody (N-Term) AP22205a
    Overlay histogram showing Hela cells stained with AP22205a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22205a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 14 - OVGP1 Antibody (N-Term) AP22205a
    AP22205a staining OVGP1 in human fallopian tube tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - OVGP1 Antibody (N-Term) AP22205a
    All lanes : Anti-OVGP1 Antibody (N-Term) at 1:2000 dilution Lane 1: human placenta lysate Lane 2: human uterus lysate Lane 3: human ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB, E
Primary Accession Q12889
Other Accession P36718
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 75421 Da
Additional info
Gene ID 5016
Other Names Oviduct-specific glycoprotein, Estrogen-dependent oviduct protein, Mucin-9, Oviductal glycoprotein, Oviductin, OVGP1, MUC9, OGP
Target/Specificity This OVGP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 107-137 amino acids from human OVGP1.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOVGP1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OVGP1
Synonyms MUC9, OGP
Function Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.
Cellular Location Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules
Tissue Location Oviduct.
Research Areas

BACKGROUND

Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.

REFERENCES

Arias E.B.,et al.Biol. Reprod. 51:685-694(1994).
Jaffe R.C.,et al.Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
Sjoeblom T.,et al.Science 314:268-274(2006).

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