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TAF4 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - TAF4 Antibody (C-Term) AP22185b
    AP22185b staining TAF4 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - TAF4 Antibody (C-Term) AP22185b
    AP22185b staining TAF4 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - TAF4 Antibody (C-Term) AP22185b
    All lanes : Anti-TAF4 Antibody (C-Term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U-251 MG whole cell lysate Lane 3: A431 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: CCRF-CEM whole cell lysate Lane 6: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB, E
Primary Accession O00268
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 110114 Da
Additional info
Gene ID 6874
Other Names Transcription initiation factor TFIID subunit 4, RNA polymerase II TBP-associated factor subunit C, TBP-associated factor 4, Transcription initiation factor TFIID 130 kDa subunit, TAF(II)130, TAFII-130, TAFII130, Transcription initiation factor TFIID 135 kDa subunit, TAF(II)135, TAFII-135, TAFII135, TAF4, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Target/Specificity This TAF4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1021-1062 amino acids from human TAF4.
Dilution IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF4 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF4
Synonyms TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Function Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.
Cellular Location Nucleus.

BACKGROUND

Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.

REFERENCES

Mengus G.,et al.Genes Dev. 11:1381-1395(1997).
Deloukas P.,et al.Nature 414:865-871(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Tanese N.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:13611-13616(1996).
Damania B.,et al.Genes Dev. 10:1369-1381(1996).

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