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PCMT1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PCMT1 Antibody (N-Term) AP22160a
    All lanes : Anti-PCMT1 Antibody (N-Term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P22061
Other Accession P15246, Q4R5H0, P23506, P80895, Q5RA89, P22062
Reactivity Human, Rat
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 24636 Da
Additional info
Gene ID 5110
Other Names Protein-L-isoaspartate(D-aspartate) O-methyltransferase, PIMT, 2.1.1.77, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, PCMT1
Target/Specificity This PCMT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 18-55 amino acids from human PCMT1.
Dilution WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCMT1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCMT1
Function Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).

REFERENCES

Ingrosso D.,et al.J. Biol. Chem. 264:20131-20139(1989).
Maclaren D.C.,et al.Biochem. Biophys. Res. Commun. 185:277-283(1992).
Takeda R.,et al.J. Biochem. 117:683-685(1995).
Shirasawa T.,et al.Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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