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SULF1 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - SULF1 Antibody (C-Term) AP22157b
    Overlay histogram showing U-251 MG cells stained with AP22157b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22157b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - SULF1 Antibody (C-Term) AP22157b
    Anti-SULF1 Antibody (C-Term) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q8IWU6
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 101027 Da
Additional info
Gene ID 23213
Other Names Extracellular sulfatase Sulf-1, hSulf-1, 3.1.6.-, SULF1, KIAA1077
Target/Specificity This SULF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 704-738 amino acids from human SULF1.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSULF1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SULF1
Synonyms KIAA1077
Function Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.
Cellular Location Endoplasmic reticulum. Golgi apparatus, Golgi stack. Cell surface Note=Also localized on the cell surface.
Tissue Location Expressed at highest levels in testis, stomach, skeletal muscle, lung, kidney, pancreas, small intestine and colon. It is also detected in normal ovarian surface epithelial cells. Down-regulation seen in ovarian carcinoma cell lines, ovarian cancers, breast, pancreatic, renal and hepatocellular carcinoma cell lines
Research Areas

BACKGROUND

Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.

REFERENCES

Morimoto-Tomita M.,et al.J. Biol. Chem. 277:49175-49185(2002).
Lai J.,et al.J. Biol. Chem. 278:23107-23117(2003).
Kikuno R.,et al.DNA Res. 6:197-205(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Chen R.,et al.J. Proteome Res. 8:651-661(2009).

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