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PI4KA Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PI4KA Antibody (Center) AP22156c
    Anti-PI4KA Antibody (Center) at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 237 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P42356
Other Accession O02811, O08662
Reactivity Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 231319 Da
Additional info
Other Names Phosphatidylinositol 4-kinase alpha, PI4-kinase alpha, PI4K-alpha, PtdIns-4-kinase alpha, 2.7.1.67, PI4KA, PIK4, PIK4CA
Target/Specificity This PI4KA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1388-1422 amino acids from the Central region of human PI4KA.
Dilution WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI4KA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PI4KA
Synonyms PIK4, PIK4CA
Function Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate.
Cellular Location Cytoplasm. Membrane. Note=Associates with the membranes of Golgi vesicles and vacuoles.
Tissue Location Expressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes.
Research Areas

BACKGROUND

Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate.

REFERENCES

Wong K.,et al.J. Biol. Chem. 269:28878-28884(1994).
Gehrmann T.,et al.Biochim. Biophys. Acta 1437:341-356(1999).
Dunham I.,et al.Nature 402:489-495(1999).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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