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FLOT2 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - FLOT2 Antibody (C-Term) AP22149b
    All lanes : Anti-FLOT2 Antibody (C-Term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: human brain lysate Lane 3: Ramos whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q14254
Other Accession A6QLR4, Q60634, Q9Z2S9
Reactivity Human, Mouse
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 47064 Da
Additional info
Gene ID 2319
Other Names Flotillin-2, Epidermal surface antigen, ESA, Membrane component chromosome 17 surface marker 1, FLOT2, ESA1, M17S1
Target/Specificity This FLOT2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 322-356 amino acids from human FLOT2.
Dilution WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFLOT2 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLOT2
Synonyms ESA1, M17S1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Cellular Location Cell membrane; Peripheral membrane protein Membrane, caveola; Peripheral membrane protein. Endosome. Note=Membrane-associated protein of caveolae
Tissue Location In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes
Research Areas

BACKGROUND

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

REFERENCES

Zody M.C.,et al.Nature 440:1045-1049(2006).
Schroeder W.T.,et al.J. Biol. Chem. 269:19983-19991(1994).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Gorbea C.,et al.J. Biol. Chem. 285:31616-31633(2010).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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