|Application ||WB, E|
|Calculated MW||33200 Da|
|Other Names||Pre-miRNA 5'-monophosphate methyltransferase, 2.1.1.-, BCDIN3 domain-containing protein, BCDIN3D|
|Target/Specificity||This BCDIN3D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from human BCDIN3D.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BCDIN3D Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||O-methyltransferase that specifically monomethylates 5'- monophosphate of cytoplasmic histidyl tRNA, acting as a capping enzyme (PubMed:28119416). Less efficiently, also methylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing (PubMed:23063121, PubMed:28119416). The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs (PubMed:23063121). There is some controversy about the methylation of pre-miR-145, since the dimethylation first described as the specific enzymatic activity cannot be reproduced by a more recent work which observes a monomehtylation of pre-miR-145 but two orders weaker than the methylation of cytosolic histidyl tRNA (PubMed:23063121, PubMed:28119416, PubMed:30127802).|
Provided below are standard protocols that you may find useful for product applications.
O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre- miRNAs.
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Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Xhemalce B.,et al.Cell 151:278-288(2012).