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Transferrin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - Transferrin Antibody AP22129a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Transferrin with AP22119a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
  • 1 - Transferrin Antibody AP22129a
    All lanes : Anti-Transferrin Antibody at 1:2000 dilution Lane 1: human plasma lysate Lane 2: human liver lysate Lane 3: human fetal liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Transferrin Antibody AP22129a
    All lanes : Anti-Transferrin Antibody at 1:2000 dilution Lane 1: human plasma lysate Lane 2: human fetal liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession P02787
Other Accession A5A6I6
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 77064 Da
Additional info
Gene ID 7018
Other Names Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF
Target/Specificity This Transferrin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-135 amino acids from human Transferrin.
Dilution IF~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTransferrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TF
Function Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Cellular Location Secreted.
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas

BACKGROUND

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

REFERENCES

Yang F.,et al.Proc. Natl. Acad. Sci. U.S.A. 81:2752-2756(1984).
Schaeffer E.,et al.Gene 56:109-116(1987).
Hershberger C.L.,et al.Ann. N. Y. Acad. Sci. 646:140-154(1991).
Beutler E.,et al.Blood 96:4071-4074(2000).
Muzny D.M.,et al.Nature 440:1194-1198(2006).

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