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alpha Tubulin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - alpha Tubulin Antibody AP22120a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling alpha Tubulin with AP22120a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
  • 4 - alpha Tubulin Antibody AP22120a
    Overlay histogram showing Hela cells stained with AP22120a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22120a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - alpha Tubulin Antibody AP22120a
    All lanes : Anti-alpha Tubulin Antibody at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, WB, E
Primary Accession P68363
Other Accession P68362, Q71U36, P68369, A5A6J1, P02550, P68370, P81947, P68361, Q4R538, P68360, P05213, Q5R1W4, Q2XVP4, Q6P9V9, Q8T6A5, P02552, P18258, P41383, Q13748, Q32KN8, P91910, Q94572, P05214, Q68FR8, P18288, P50719, P02553, P36220
Reactivity Human
Predicted Bovine, Chicken, Human, Mouse, Pig, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 50152 Da
Additional info
Gene ID 10376
Other Names Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B
Target/Specificity This alpha Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 408-439 amino acids from human alpha Tubulin.
Dilution IF~~1:25
FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsalpha Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBA1B
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

REFERENCES

Cowan N.J.,et al.Mol. Cell. Biol. 3:1738-1745(1983).
de Hostos E.L.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Li Q.,et al.Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.

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