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RPS4X Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - RPS4X Antibody (C-Term) AP22116b
    Overlay histogram showing Hela cells stained with AP22116b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22116b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - RPS4X Antibody (C-Term) AP22116b
    All lanes : Anti-RPS4X Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: rat lung lysate Lane 6: mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P62701
Other Accession Q76N24, P62705, Q76MY1, P62704, O62738, P62702, P62703, O62739, P79103, P47836, P47961, P49401, Q6PBC4
Reactivity Human, Mouse, Rat
Predicted Bovine, Chicken, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 29598 Da
Additional info
Gene ID 6191
Other Names 40S ribosomal protein S4, X isoform, SCR10, Single copy abundant mRNA protein, RPS4X, CCG2, RPS4, SCAR
Target/Specificity This RPS4X antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 209-243 amino acids from the human region of human RPS4X.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS4X Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS4X
Synonyms CCG2, RPS4, SCAR
Cellular Location Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs

REFERENCES

Fisher E.M.C.,et al.Cell 63:1205-1218(1990).
Watanabe M.,et al.J. Cell Sci. 100:35-43(1991).
Zuo L.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Dmitrenko V.V.,et al.Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.

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