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COXIV Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - COXIV Antibody AP22111a
    Overlay histogram showing NIH/3T3 cells stained with AP22111a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22111a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 4 - COXIV Antibody AP22111a
    Overlay histogram showing NIH/3T3 cells stained with AP22111a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22111a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - COXIV Antibody AP22111a
    All lanes : Anti-COXIV Antibody at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: BA/F3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P19783
Other Accession P10888
Reactivity Human, Mouse, Rat
Predicted Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 19530 Da
Additional info
Gene ID 12857
Other Names Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a
Target/Specificity This COXIV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-169 amino acids from the mouse region of human COXIV.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOXIV Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cox4i1
Synonyms Cox4, Cox4a
Function This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Cellular Location Mitochondrion inner membrane.
Research Areas

BACKGROUND

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

REFERENCES

Grossman L.I.,et al.Nucleic Acids Res. 18:6454-6454(1990).
Carter R.S.,et al.Arch. Biochem. Biophys. 288:97-106(1991).
Carninci P.,et al.Science 309:1559-1563(2005).
Lubec G.,et al.Submitted (APR-2007) to UniProtKB.
Park J.,et al.Mol. Cell 50:919-930(2013).

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