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beta II Tubulin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - beta II Tubulin Antibody AP22106a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (mouse myoblast cell line) cells labeling beta II Tubulin with AP22106a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on C2C12 cell line. The nuclear counter stain is DAPI (blue).
  • 4 - beta II Tubulin Antibody AP22106a
    Overlay histogram showing NIH/3T3 cells stained with AP22106a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22106a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 4 - beta II Tubulin Antibody AP22106a
    Overlay histogram showing NIH/3T3 cells stained with AP22106a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22106a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - beta II Tubulin Antibody AP22106a
    All lanes : Anti-beta II Tubulin at 1:8000 dilution Lane 1: mouse brain lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: PC-12 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, WB, E
Primary Accession Q7TMM9
Other Accession P09203, Q9NFZ7, Q13885, Q4R5B3, P85108, Q6B856, Q9BVA1, Q9CWF2, Q3KRE8, P32882, P13602, Q9NFZ5, P30156, P20802, O59837, P02554
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Rat, Pig, Chicken, Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 22151
Other Names Tubulin beta-2A chain, Tubb2a, Tubb2
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-46 amino acids from human.
Dilution IF~~1:25
FC~~1:25
WB~~1:8000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsbeta II Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tubb2a
Synonyms Tubb2
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Cellular Location Cytoplasm, cytoskeleton.
Research Areas

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

REFERENCES

Carninci P.,et al.Science 309:1559-1563(2005).
Lubec G.,et al.Submitted (JAN-2009) to UniProtKB.
Janke C.,et al.Science 308:1758-1762(2005).
Rogowski K.,et al.Cell 137:1076-1087(2009).
Yoshida K.,et al.Biochem. Biophys. Res. Commun. 389:506-511(2009).

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