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RPS27 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - RPS27 Antibody (N-Term) AP22097a
    AP22097a staining RPS27 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 4 - RPS27 Antibody (N-Term) AP22097a
    Overlay histogram showing Jurkat cells stained with AP22097a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22097a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - RPS27 Antibody (N-Term) AP22097a
    All lanes : Anti-RPS27 Antibody (N-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 9 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P42677
Other Accession Q2KHT7, Q6ZWU9, Q71TY3
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 9461 Da
Additional info
Gene ID 6232
Other Names 40S ribosomal protein S27, Metallopan-stimulin 1, MPS-1, RPS27, MPS1
Target/Specificity This RPS27 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-37 amino acids from human RPS27.
Dilution IHC-P~~1:25
FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS27 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS27
Synonyms MPS1
Tissue Location Expressed in a wide variety of actively proliferating cells and tumor tissues

REFERENCES

Fernandez-Pol J.A.,et al.J. Biol. Chem. 268:21198-21204(1993).
Tsui S.K.W.,et al.Biochem. Mol. Biol. Int. 40:611-616(1996).
Yoshihama M.,et al.Genome Res. 12:379-390(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).

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