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PIGC Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - PIGC Antibody (C-Term) AP22091b
    Overlay histogram showing K562 cells stained with AP22091b (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22091b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - PIGC Antibody (C-Term) AP22091b
    All lanes : Anti-PIGC Antibody (C-Term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q92535
Other Accession Q3ZBX1, Q9CXR4, Q5PQQ4
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 33583 Da
Additional info
Gene ID 5279
Other Names Phosphatidylinositol N-acetylglucosaminyltransferase subunit C, 2.4.1.198, Phosphatidylinositol-glycan biosynthesis class C protein, PIG-C, PIGC, GPI2
Target/Specificity This PIGC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 264-294 amino acids from human PIGC.
Dilution FC~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIGC Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIGC
Synonyms GPI2
Function Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Research Areas

BACKGROUND

Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.

REFERENCES

Inoue N.,et al.Biochem. Biophys. Res. Commun. 226:193-199(1996).
Hong Y.,et al.Genomics 44:347-349(1997).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).

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