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ITIH4 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - ITIH4 Antibody (C-Term) AP22074b
    Overlay histogram showing U-2OS cells stained with AP22074b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22074b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ITIH4 Antibody (C-Term) AP22074b
    Anti-ITIH4 Antibody (C-Term) at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q14624
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 103357 Da
Additional info
Gene ID 3700
Other Names Inter-alpha-trypsin inhibitor heavy chain H4, ITI heavy chain H4, ITI-HC4, Inter-alpha-inhibitor heavy chain 4, Inter-alpha-trypsin inhibitor family heavy chain-related protein, IHRP, Plasma kallikrein sensitive glycoprotein 120, Gp120, PK-120, 70 kDa inter-alpha-trypsin inhibitor heavy chain H4, 35 kDa inter-alpha-trypsin inhibitor heavy chain H4, ITIH4, IHRP, ITIHL1, PK120
Target/Specificity This ITIH4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 885-917 amino acids from human ITIH4.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsITIH4 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ITIH4
Synonyms IHRP, ITIHL1, PK120
Function Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
Cellular Location Secreted.
Tissue Location Liver specific.
Research Areas

BACKGROUND

Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.

REFERENCES

Nishimura H.,et al.FEBS Lett. 357:207-211(1995).
Saguchi K.,et al.J. Biochem. 117:14-18(1995).
Saguchi K.,et al.J. Biochem. 119:898-905(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kroczynska B.,et al.Biochem. Biophys. Res. Commun. 338:1467-1477(2005).

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