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SH3PXD2B Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - SH3PXD2B Antibody (C-Term) AP22070b
    Overlay histogram showing U-2OS cells stained with AP22070b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22070b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - SH3PXD2B Antibody (C-Term) AP22070b
    All lanes : Anti-SH3PXD2B Antibody (C-Term) at 1:2000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: U-87 MG whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession A1X283
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 101579 Da
Additional info
Gene ID 285590
Other Names SH3 and PX domain-containing protein 2B, Adapter protein HOFI, Factor for adipocyte differentiation 49, Tyrosine kinase substrate with four SH3 domains, SH3PXD2B, FAD49, KIAA1295, TKS4
Target/Specificity This SH3PXD2B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 596-628 amino acids from human SH3PXD2B.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSH3PXD2B Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SH3PXD2B
Synonyms FAD49, KIAA1295, TKS4
Function Adapter protein involved in invadopodia and podosome formation and extracellular matrix degradation. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. Plays a role in mitotic clonal expansion during the immediate early stage of adipocyte differentiation (By similarity).
Cellular Location Cytoplasm. Cell projection, podosome. Note=Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.
Tissue Location Expressed in fibroblasts.
Research Areas

BACKGROUND

Adapter protein involved in invadopodia and podosome formation and extracellular matrix degradation. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. Plays a role in mitotic clonal expansion during the immediate early stage of adipocyte differentiation (By similarity).

REFERENCES

Hishida T.,et al.FEBS J. 275:5576-5588(2008).
Lanyi A.,et al.Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
Schmutz J.,et al.Nature 431:268-274(2004).
Nagase T.,et al.DNA Res. 7:65-73(2000).
Abram C.L.,et al.J. Biol. Chem. 278:16844-16851(2003).

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